Crystal Thomas has over 25 years of experience conducting HPLC pigment analysis for ocean color research and researching method improvements. Thomas's research interests include method development and quality assurance/quality control, particularly as they relate to identifying and minimizing sources of uncertainty in HPLC pigment measurements. Currently, she manages the activities of GSFC’s Ocean Ecology Laboratory HPLC pigment analysis program.
Scientific lead on NASA contract to analyze HPLC phytoplankton pigment samples for various PIs in support of ocean color research, and research into analysis of water-soluble phytoplankton pigments (phycobilins) and advanced chromatographic techniques (LC-MS, UHPLC).
Scientific lead on NASA contract to analyze HPLC pigment samples for various PIs in support of ocean color research
Classroom and laboratory instructor for short course, Phytoplankton Bio-Optical Variability in Coastal Systems. Taught students how to establish and maintain an HPLC pigment analysis laboratory, with emphasis on Quality Assurance/Quality Control. Sponsored by NF-POGO (Nippon Foundation-Partnership for Observation of the Global Oceans) and AWI-CofE (Alfred-Wegener Institute Center for Excellence), 19-23 January, 2015, Ensendada, Mexico
Hydrospheric and Biospheric Sciences (HOBI) Award for Scientific/Technical Support (2015)
Neeley, A. R., M. W. Lomas, A. Mannino, C. Thomas, and R. Vandermeulen. 2022. "Impact of growth phase, pigment adaptation and climate change conditions on the cellular pigment and carbon content of fifty‐one phytoplankton isolates." Journal of Phycology, [10.1111/jpy.13279]
Valente, A., S. Sathyendranath, V. Brotas, et al. S. Groom, M. Grant, T. Jackson, A. Chuprin, M. Taberner, R. Airs, D. Antoine, R. Arnone, W. M. Balch, K. Barker, R. Barlow, S. Bélanger, J.-F. Berthon, Ş. Beşiktepe, Y. Borsheim, A. Bracher, V. Brando, R. J. Brewin, E. Canuti, F. P. Chavez, A. Cianca, H. Claustre, L. Clementson, R. Crout, A. Ferreira, S. Freeman, R. Frouin, C. García-Soto, S. W. Gibb, R. Goericke, R. Gould, N. Guillocheau, S. B. Hooker, C. Hu, M. Kahru, M. Kampel, H. Klein, S. Kratzer, R. Kudela, J. Ledesma, S. Lohrenz, H. Loisel, A. Mannino, V. Martinez-Vicente, P. Matrai, D. McKee, B. G. Mitchell, T. Moisan, E. Montes, F. Muller-Karger, A. Neeley, M. Novak, L. O'Dowd, M. Ondrusek, T. Platt, A. J. Poulton, M. Repecaud, R. Röttgers, T. Schroeder, T. Smyth, D. Smythe-Wright, H. M. Sosik, C. Thomas, R. Thomas, G. Tilstone, A. Tracana, M. Twardowski, V. Vellucci, K. Voss, J. Werdell, M. Wernand, B. Wojtasiewicz, S. Wright, and G. Zibordi. 2022. "A compilation of global bio-optical in situ data for ocean-colour satellite applications – version three." Earth System Science Data, [10.5194/essd-2022-159]
Catlett, D., D. Siegel, R. Simons, et al. N. Guillocheau, F. Henderikx-Freitas, and C. Thomas. 2021. "Diagnosing seasonal to multi-decadal phytoplankton group dynamics in a highly productive coastal ecosystem." Progress in Oceanography, 197: 102637 [10.1016/j.pocean.2021.102637]
Sahoo, S., S. K. Baliarsingh, A. A. Lotliker, et al. U. K. Pradhan, C. S. Thomas, and K. Sahu. 2017. "Effect of physico-chemical regimes and tropical cyclones on seasonal distribution of chlorophyll-a in the Chilika Lagoon, east coast of India." Environmental Monitoring and Assessment, 189 (4): 153 [10.1007/s10661-017-5850-5]
Chaves, J. E., J. Werdell, C. W. Proctor, et al. A. R. Neeley, S. A. Freeman, C. S. Thomas, and S. B. Hooker. 2015. "Assessment of ocean color data records from MODIS-Aqua in the western Arctic Ocean." Deep Sea Research Part II: Topical Studies in Oceanography, 118 (Part A): 32-43 [10.1016/j.dsr2.2015.02.011]
Egeland, E. J., J. Garrido, L. Clementson, et al. K. Andresen, C. S. Thomas, M. Zapata, R. Airs, C. Llewellyn, G. Newman, R. Rodriguez, and S. Roy. 2011. "Part VII: Data sheets aiding identification of phytoplankton carotenoids and chlorophylls." Phytoplankton Pigments: Characterization, Chemotaxonomy, and Applications in Oceanography, Cambridge, New York: 665-674, ISBN: 978-1-107-00066-7. [10.1017/CBO9780511732263]
Neeley, A. R., C. S. Thomas, S. B. Hooker, and L. Van Heukelem. 2011. "HPLC instrument performance metrics and validation." Phytoplankton Pigments: Characterization, Chemotaxonomy, and Applications in Oceanography, New York: Cambridge University Press, 890, ISBN: 978-1-107-00066-7.
Claustre, H., S. B. Hooker, L. Van Heukelem, et al. J.-F. Berthon, R. Barlow, J. Ras, H. Sessions, C. Targa, C. S. Thomas, D. van der Linde, and J.-C. Marty. 2004. "An intercomparison of HPLC phytoplankton pigment methods using in situ samples: application to remote sensing and database activities." Marine Chem, 85 (1-2): 41-61 [10.1016/j.marchem.2003.09.002]
Van Heukelem, L., and C. Thomas. 2001. "Computer-assisted high-performance liquid chromatography method development with applications to the isolation and analysis of phytoplankton pigments." J. Chrom. A., 910: 31-49.
Chaves Cedeno, J. E., P. J. Werdell, C. W. Proctor, et al. A. R. Neeley, S. A. Freeman, C. S. Thomas, and S. B. Hooker. 2014. "Assessment of ocean color data records from MODIS-Aqua in the western Arctic Ocean." Proc. Ocean Optics XXII
Hooker, S. B., L. Clementson, C. S. Thomas, et al. L. Schluter, M. Allerup, J. Ras, H. Claustre, C. Normandeau, J. Cullen, M. Kienast, W. Kozlowski, M. Vernet, S. Chakraborty, S. Lohrenz, M. Tuel, D. Redalje, P. Cartaxana, C. R. Mendes, V. Brotas, S. G. Matondkar, S. G. Parab, A. R. Neeley, and E. S. Egeland. 2012. "The Fifth SeaWiFS HPLC Analysis Round-Robin Experiment (SeaHARRE-5)." NASA Technical Memo. 2012-217503 NASA Goddard Space Flight Center, Greenbelt, Maryland, 98.
Hooker, S. B., C. S. Thomas, L. Van Heukelem, et al. L. Schluter, M. E. Russ, J. Ras, H. Claustre, L. Clementson, E. Canuti, J.-F. Berthon, J. Perl, C. Normandeau, J. Cullen, M. Kienast, and J. L. Pinckney. 2010. "The Fourth SeaWiFS HPLC Analysis Round-Robin Experiment (SeaHARRE-4)." NASA Technical Memo 2010-215857 NASA Goddard Space Flight Center, Greenbelt, Maryland, 75.
Hooker, S. B., L. Van Heukelem, C. S. Thomas, et al. H. Claustre, J. Ras, L. Schluter, L. Clementson, D. van der Linde, E. Eker-Develi, J.-F. Berthon, R. Barlow, H. Sessions, H. Ismail, and J. Perl. 2009. "The Third SeaWiFS HPLC Analysis Round-Robin Experiment (SeaHARRE-3)." NASA Technical Memo 2009-215849 NASA Goddard Space Flight Center, Greenbelt, Maryland, 97.
Hooker, S. B., V. Stuart, C. Trees, et al. J. Perl, L. Schlter, L. V. Heukelem, C. S. Thomas, R. Barlow, J. Ras, H. Claustre, H. Sessions, E. Head, L. Clementson, J. Fishwick, C. Llewellyn, and J. Aiken. 2005. "The Second SeaWiFS HPLC Analysis Round-Robin Experiment (SeaHARRE-2)." NASA Tech Memo 2005-212785 NASA Goddard Space Flight Center, Greenbelt, Maryland, 112 pp.
Classroom and laboratory instructor for short course - Phytoplankton bio-optical variability:
application to the study of coastal systems.
part of a short course, Phytoplankton Bio-Optical Variability in Coastal Systems, taught students how to establish and maintain an HPLC pigment analysis laboratory, with emphasis on Quality Assurance/Quality Control, sponsored by NF-POGO (Nippon Foundation-Partnership for Observation of the Global Oceans) and AWI-CofE (Alfred-Wegener Institute Center for Excellence), 19-23 January, 2015, Ensendada, Mexico
Pigment Analysis: Sample Collection and Measurement Techniques
10 / 21 / 2013NANO (NF-POGO Alumni Network for Oceans) meeting, Fundaction la Salle, Venezuela
Components of High-Quality HPLC Measurements
10 / 22 / 2013NANO (NF-POGO Alumni Network for Oceans) meeting, Fundaction la Salle, Venezuela
HPLC Methods and Intercomparison
10 / 22 / 2013NANO (NF-POGO Alumni Network for Oceans) meeting, Fundaction la Salle, Venezuela
Thomas, C., J. Chaves, A. Mannino (2018), Global variability of phytoplankton photosynthetic pigments determined by High Performance Liquid Chromatography (HPLC): a precision assessment of the dataset analyzed by NASA, Abstract OD34A-2752, presented at 2018 Ocean Sciences Meeting, Portland, OR, 12-16 Feb.
2 / 14 / 2018Crystal Thomas has over 25 years of experience conducting HPLC pigment analysis for ocean color research and researching method improvements. Thomas's research interests include method development and quality assurance/quality control, particularly as they relate to identifying and minimizing sources of uncertainty in HPLC pigment measurements. Currently, she manages the activities of GSFC’s Ocean Ecology Laboratory HPLC pigment analysis program.
Neeley, A. R., M. W. Lomas, A. Mannino, C. Thomas, and R. Vandermeulen. 2022. "Impact of growth phase, pigment adaptation and climate change conditions on the cellular pigment and carbon content of fifty‐one phytoplankton isolates." Journal of Phycology [10.1111/jpy.13279]
Valente, A., S. Sathyendranath, V. Brotas, et al. S. Groom, M. Grant, T. Jackson, A. Chuprin, M. Taberner, R. Airs, D. Antoine, R. Arnone, W. M. Balch, K. Barker, R. Barlow, S. Bélanger, J.-F. Berthon, Ş. Beşiktepe, Y. Borsheim, A. Bracher, V. Brando, R. J. Brewin, E. Canuti, F. P. Chavez, A. Cianca, H. Claustre, L. Clementson, R. Crout, A. Ferreira, S. Freeman, R. Frouin, C. García-Soto, S. W. Gibb, R. Goericke, R. Gould, N. Guillocheau, S. B. Hooker, C. Hu, M. Kahru, M. Kampel, H. Klein, S. Kratzer, R. Kudela, J. Ledesma, S. Lohrenz, H. Loisel, A. Mannino, V. Martinez-Vicente, P. Matrai, D. McKee, B. G. Mitchell, T. Moisan, E. Montes, F. Muller-Karger, A. Neeley, M. Novak, L. O'Dowd, M. Ondrusek, T. Platt, A. J. Poulton, M. Repecaud, R. Röttgers, T. Schroeder, T. Smyth, D. Smythe-Wright, H. M. Sosik, C. Thomas, R. Thomas, G. Tilstone, A. Tracana, M. Twardowski, V. Vellucci, K. Voss, J. Werdell, M. Wernand, B. Wojtasiewicz, S. Wright, and G. Zibordi. 2022. "A compilation of global bio-optical in situ data for ocean-colour satellite applications – version three." Earth System Science Data [10.5194/essd-2022-159]
Catlett, D., D. Siegel, R. Simons, et al. N. Guillocheau, F. Henderikx-Freitas, and C. Thomas. 2021. "Diagnosing seasonal to multi-decadal phytoplankton group dynamics in a highly productive coastal ecosystem." Progress in Oceanography 197 102637 [10.1016/j.pocean.2021.102637]
Sahoo, S., S. K. Baliarsingh, A. A. Lotliker, et al. U. K. Pradhan, C. S. Thomas, and K. Sahu. 2017. "Effect of physico-chemical regimes and tropical cyclones on seasonal distribution of chlorophyll-a in the Chilika Lagoon, east coast of India." Environmental Monitoring and Assessment 189 (4): 153 [10.1007/s10661-017-5850-5]
Chaves, J. E., J. Werdell, C. W. Proctor, et al. A. R. Neeley, S. A. Freeman, C. S. Thomas, and S. B. Hooker. 2015. "Assessment of ocean color data records from MODIS-Aqua in the western Arctic Ocean." Deep Sea Research Part II: Topical Studies in Oceanography 118 (Part A): 32-43 [10.1016/j.dsr2.2015.02.011]
Egeland, E. J., J. Garrido, L. Clementson, et al. K. Andresen, C. S. Thomas, M. Zapata, R. Airs, C. Llewellyn, G. Newman, R. Rodriguez, and S. Roy. 2011. "Part VII: Data sheets aiding identification of phytoplankton carotenoids and chlorophylls." Phytoplankton Pigments: Characterization, Chemotaxonomy, and Applications in Oceanography 665-674 [10.1017/CBO9780511732263]
Neeley, A. R., C. S. Thomas, S. B. Hooker, and L. Van Heukelem. 2011. "HPLC instrument performance metrics and validation." Phytoplankton Pigments: Characterization, Chemotaxonomy, and Applications in Oceanography 890
Claustre, H., S. B. Hooker, L. Van Heukelem, et al. J.-F. Berthon, R. Barlow, J. Ras, H. Sessions, C. Targa, C. S. Thomas, D. van der Linde, and J.-C. Marty. 2004. "An intercomparison of HPLC phytoplankton pigment methods using in situ samples: application to remote sensing and database activities." Marine Chem 85 (1-2): 41-61 [10.1016/j.marchem.2003.09.002]
Van Heukelem, L., and C. Thomas. 2001. "Computer-assisted high-performance liquid chromatography method development with applications to the isolation and analysis of phytoplankton pigments." J. Chrom. A. 910 31-49
Chaves Cedeno, J. E., P. J. Werdell, C. W. Proctor, et al. A. R. Neeley, S. A. Freeman, C. S. Thomas, and S. B. Hooker. 2014. "Assessment of ocean color data records from MODIS-Aqua in the western Arctic Ocean." Proc. Ocean Optics XXII
Hooker, S. B., L. Clementson, C. S. Thomas, et al. L. Schluter, M. Allerup, J. Ras, H. Claustre, C. Normandeau, J. Cullen, M. Kienast, W. Kozlowski, M. Vernet, S. Chakraborty, S. Lohrenz, M. Tuel, D. Redalje, P. Cartaxana, C. R. Mendes, V. Brotas, S. G. Matondkar, S. G. Parab, A. R. Neeley, and E. S. Egeland. 2012. "The Fifth SeaWiFS HPLC Analysis Round-Robin Experiment (SeaHARRE-5)." NASA Technical Memo. 2012-217503 98
Hooker, S. B., C. S. Thomas, L. Van Heukelem, et al. L. Schluter, M. E. Russ, J. Ras, H. Claustre, L. Clementson, E. Canuti, J.-F. Berthon, J. Perl, C. Normandeau, J. Cullen, M. Kienast, and J. L. Pinckney. 2010. "The Fourth SeaWiFS HPLC Analysis Round-Robin Experiment (SeaHARRE-4)." NASA Technical Memo 2010-215857 75
Hooker, S. B., L. Van Heukelem, C. S. Thomas, et al. H. Claustre, J. Ras, L. Schluter, L. Clementson, D. van der Linde, E. Eker-Develi, J.-F. Berthon, R. Barlow, H. Sessions, H. Ismail, and J. Perl. 2009. "The Third SeaWiFS HPLC Analysis Round-Robin Experiment (SeaHARRE-3)." NASA Technical Memo 2009-215849 97
Hooker, S. B., V. Stuart, C. Trees, et al. J. Perl, L. Schlter, L. V. Heukelem, C. S. Thomas, R. Barlow, J. Ras, H. Claustre, H. Sessions, E. Head, L. Clementson, J. Fishwick, C. Llewellyn, and J. Aiken. 2005. "The Second SeaWiFS HPLC Analysis Round-Robin Experiment (SeaHARRE-2)." NASA Tech Memo 2005-212785 112 pp